LOCUS       40924_13775        6652 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Yeast two-hybrid vector pCtrp DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Banno H.
  TITLE     Identification of proteins that interact with a plant nuclear 
            protein using the yeast split-Trp sensor
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Banno H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2014) Contact:Hiroharu Banno Chubu University, 
            Bioscience and Biotechnology; 1200 Matsumoto-cho, Kasugai, Aichi 
            487-8501, Japan Phone :81-568-52-6242
REFERENCE   3  (bases 1 to 6652)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6652)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-APR-2014) Contact:Hiroharu Banno Chubu University, Bioscience 
            and Biotechnology"; volume: " 1200 Matsumoto-cho, Kasugai, Aichi 
            487-8501, Japan Phone "; pages: "81-568-52-624"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..40
                     /label=multiple cloning site(MCS)
                     /note="multiple cloning site(MCS)"
     CDS             41..70
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             77..106
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             113..142
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             149..178
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      758..1005
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1253..1841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2007..2663)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(3111..3911)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3912..4132)
                     /label=URA3 promoter
     rep_origin      complement(4731..5611)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(5622..6135)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6154..6595
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        6627..6645
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aagcttggcg cgcccctagg ctcgaggcgg ccgcggatcc gaacagaaat taatctctga
       61 agaggattta agaaatgagc aaaagttaat ctcagaggaa gaccttcgta acgaacaaaa
      121 gttaatctca gaggaggact tacggaacga gcagaaactt atctctgaag aagaccttaa
      181 gagaacaatt gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag catataaaaa
      241 tagttcaggc actccgaaat acttggttgg cgtgtttcgt aatcaaccta aggaggatgt
      301 tttggctctg gtcaatgatt acggcattga tatcgtccaa ctgcatggag atgagtcgtg
      361 gcaagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat ttccaaaaga
      421 ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc ccttgtttga
      481 ttcagaagca ggtgggacag gtgaactttt ggattggaac tcgatttctg actgggttgg
      541 aaggcaagag agccccgaaa gcttacattt tatgttagct ggtggactga cgccagaaaa
      601 tgttggtgat gcgcttagat taaatggcgt tattggtgtt gatgtaagcg gaggtgtgga
      661 gacaaatggt gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg ctaagaaata
      721 gctcgactaa ttgaataata agctctagag ggccgcatca tgtaattagt tatgtcacgc
      781 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
      841 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata
      901 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa
      961 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcaagct gcggccctgc
     1021 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1081 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1141 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1201 caaaaggcca gcaaaagccc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1261 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1321 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1381 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1441 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1501 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1561 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1621 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1681 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagct
     1921 tgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctagg
     1981 caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     2041 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     2101 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     2161 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     2221 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     2281 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     2341 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     2401 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     2461 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     2521 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     2581 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     2641 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     2701 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttaccaag
     2761 ctcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2821 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2881 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2941 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3001 ttgaatactc atactcttcc tttttcaatg ggtaataact gatataatta aattgaagct
     3061 ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta gttttgctgg
     3121 ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta
     3181 gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag
     3241 accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc
     3301 acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga
     3361 gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta
     3421 tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta
     3481 ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc
     3541 acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac
     3601 tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg
     3661 tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag
     3721 atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt
     3781 aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg
     3841 atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta
     3901 gctttcgaca tgatttatct tcgtttcctg caggtttttg ttctgtgcag ttgggttaag
     3961 aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt
     4021 ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa
     4081 agaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg aaaagctagc
     4141 ttatcgatga taagctgtca aagatgagaa ttaattccac ggactataga ctatactaga
     4201 tactccgtct actgtacgat acacttccgc tcaggtcctt gtcctttaac gaggccttac
     4261 cactcttttg ttactctatt gatccagctc agcaaaggca gtgtgatcta agattctatc
     4321 ttcgcgatgt agtaaaacta gctagaccga gaaagagact agaaatgcaa aaggcacttc
     4381 tacaatggct gccatcatta ttatccgatg tgacgctgca gcttctcaat gatattcgaa
     4441 tacgctttga ggagatacag cctaatatcc gacaaactgt tttacagatt tacgatcgta
     4501 cttgttaccc atcattgaat tttgaacatc cgaacctggg agttttccct gaaacagata
     4561 gtatatttga acctgtataa taatatatag tctagcgctt tacggaagac aatgtatgta
     4621 tttcggttcc tggagaaact attgcatcta ttgcataggt aatcttgcac gtcgcatccc
     4681 cggttcattt tctgcgtttc catcttgcac ttcaatagca tatctttgtt aacgaagcat
     4741 ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     4801 atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa
     4861 gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg acgagagcgc taatttttca
     4921 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta
     4981 ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt
     5041 ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc
     5101 ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat
     5161 tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc
     5221 tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt
     5281 gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta
     5341 tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt
     5401 attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat
     5461 actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa
     5521 ggtggatggg taggttatat agggatatag cacagagata tatagcaaag agatactttt
     5581 gagcaatgtt tgtggaagcg gtattcgcaa tgggaagctc caccccggtt gataatcaga
     5641 aaagccccaa aaacaggaag attgtataag caaatattta aattgtaaac gttaatattt
     5701 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaacgaa tagcccgaaa
     5761 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     5821 tttccaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaggg
     5881 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     5941 ggtgccgtaa agcagtaaat cggaagggta aacggatgcc cccatttaga gcttgacggg
     6001 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggggctaggg
     6061 cggtgggaag tgtaggggtc acgctgggcg taaccaccac acccgccgcg cttaatgggg
     6121 cgctacaggg cgcgtgggga tgatccacta gtacggatta gaagccgccg agcgggtgac
     6181 agccctccga aggaagactc tcctccgtgc gtcctcgtct tcaccggtcg cgttcctgaa
     6241 acgcagatgt gcctcgcgcc gcactgctcc gaacaataaa gattctacaa tactagcttt
     6301 tatggttatg aagaggaaaa attggcagta acctggcccc acaaaccttc aaatgaacga
     6361 atcaaattaa caaccatagg atgataatgc gattagtttt ttagccttat ttctggggta
     6421 attaatcagc gaagcgatga tttttgatct attaacagat atataaatgc aaaaactgca
     6481 taaccacttt aactaatact ttcaacattt tcggtttgta ttacttctta ttcaaatgta
     6541 ataaaagtat caacaaaaaa ttgttaatat acctctatac tttaacgtca aggagaaaaa
     6601 accccggatc ggactactag cagctgtaat acgactcact atagggaata tt
//
